Antibody Fragmentation
Although the Fc region of an antibody molecule is essential for its in vivo functionality, the same is not necessarily true in diagnostic applications where it can sometimes contribute to non-specific binding and background issues. Fleet Bioprocessing Ltd. are expert in enzymic fragmentation procedures, yielding Fab or F(ab’) fragments which can sometimes offer improved performance.
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Contact us via our enquiry form here if you would like to discuss an antibody fragmentation project.
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Example Projects
Antibody fragments improve assay backgrounds in food pathogen ELISAs
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Over the last two decades, Fleet have developed a wide expertise in antibody fragmentation by enzymatic digestion to facilitate the production of antibody fragments ((Fab')2 , Fab, etc), and their subsequent conjugation for diagnostic applications. A UK-based food testing company observed significantly improved backgrounds by employing Fleet antibody fragments in their microtitre plate-based pathogen assays.
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Routine antibody fragmentations under GMP conditions
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Fleet routinely manufacture antibody fragments under GMP standards for use in regulated IVD products, where high levels of process definition, reproducibility and documentation are critical. Reproducibly good assay performance has been observed over multiple fragmentation batches.
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Long-term R&D project support
Over the last 24-months, Fleet has supported a UK-based diagnostics company in their R&D activities to empower thier development of a cardiac biomarker assay with unparalleled sensitivity. This support has comprised a wide range of activities from development of Ab fragmentation approaches to the optimisation of Ab-fluorophore and other Ab-protein conjugations.
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