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Oligonucleotide & Aptamer Conjugates

Fleet Bioprocessing Ltd. have plenty of experience attaching antibodies, streptavidin and other proteins to oligonucleotides.

Generally we will couple oligos via an amine or thiol group incorporated at the 3’- or 5’- terminal, allowing us to use highly reproducible heterobifunctional coupling chemistry to prepare your conjugate.

In bioconjugation terms, aptamers simply behave like big oligos – although the purification strategy may differ, similar chemical approaches can be used.

Contact us via our enquiry form here to discuss labelling your oligo or aptamer.

Nucleic acid

Example Projects

Antibody-oligo conjugates for Dutch biotech company

Working with a Dutch biotech company, Fleet have formulated numerous antibody-oligonucleotide conjugates. Using their expertise in the handling, conjugation and purification of this type of reagent, Fleet have developed effective methods to link the oligos to the target antibody via terminal functional groups such as protected sulfhydryls. Since developing these methods, Fleet has formulated over 50 different antibody-oligo and antibody-morpholino combinations targeting specific antibody oligo ratios (OAR). To complement their synthesis Fleet were able to develop SDS- PAGE methods to help characterise these conjugates.

Novel aptamer linker design for lateral flow applications

A start-up company wanted to link aptamers to novel targets, to allow detection of infectious diseases. We came up with a novel linker reagent design to meet this hitherto unexplored need.

Peptide-morpholino conjugates for a Canadian research group

Working with a prestigious university group in Quebec, Fleet have developed coupling chemistry that modifies their proprietary morpholino and reacts this with an engineered peptide for bioorthogonal conjugation of the two components. This method has been shown to be easily scalable to 10's mgs and reproducible across multiple batches and morpholinos.

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