Antibody-Enzyme Conjugates & Other Enzyme-Labelling Applications
With the use of suitable substrates, enzymes such as horseradish peroxidase (HRP) and alkaline phosphatase (AP) can be exploited in colorimetric, fluorescent and luminescent signal generation approaches, capable of very high sensitivity. These approaches are employed in a very wide range of applications, including commercial immunoassays, cytochemical and histochemical procedures, Western blots, etc.
Antibody-HRP and antibody-AP conjugates form some of Fleet Bioprocessing Ltd.’s most popular product categories, and our expertise in optimising these conjugation procedures is unparalleled. We use “clean” heterobifunctional coupling techniques which give a much more homogeneous product than older chemistries. We typically use high-resolution size exclusion chromatography (HR-SEC) to purify our enzyme conjugates, the exact choice of purification conditions often being a key factor in obtaining optimal conjugate performance. Fleet’s knowhow will always make sure that you get the best out of your enzyme-labelled antibodies!
Of course we regularly couple HRP and other enzymes to targets other than antibodies, and have made countless conjugates between enzymes and small molecules, protein antigens, streptavidin, oligonucleotides, etc.
Contact us via our enquiry form here to discuss your enzyme conjugation requirements.
Example Projects
Development of mAb-HRP conjugates for immunoassay
Fleet carried out a program of work to develop monoclonal-HRP conjugates giving excellent performance in the customer's assay (high signal, minimum non-specific binding). The conjugation has been validated for multiple batches of key raw materials and for a range of production scales.
Antibody-alkaline phosphatase conjugate for TB diagnostic
Fleet developed a panel of monoclonal antibody-alkaline phosphatase conjugates for use in a novel TB test. Full robustness studies were executed by Fleet to confirm that all key variables were under control.