Biotin & Streptavidin Conjugates
Due to its effectively irreversible nature, the biotin-avidin interaction has found countless applications since its discovery in the 1960s.
Biotin conjugates of antibodies and other proteins form one of Fleet Bioprocessing Ltd.’s most popular product categories – we’re making these week in, week out, and have the knowhow to ensure we optimise performance for your application.
We have learnt that the optimum biotin incorporation – which we always aim to measure using a colorimetric test procedure – is not always easy to predict. Therefore we often prepare optimisation panels at a small scale, allowing conjugates with 3 – 5 different incorporation levels to be quickly compared.
Streptavidin is the most commonly-encountered avidin analogue in use, and streptavidin-labelled proteins are very useful reagents, for example enabling signal-amplification approaches with exquisite sensitivity.
Contact us via our enquiry form here to discuss your biotin or streptavidin-labelling requirements.
.jpg)
Example Projects
Biotin and streptavidin enzyme conjugates for high-sensitivity immunoassay applications
Fleet have developed biotin-HRP and streptavidin-HRP detection conjugates for a number of customer applications, typically allowing signal amplification for sensitive ELISA applications.
Biotinylation of "problem" antigens.
Working for a global leader in peptide synthesis and bioanalysis, Fleet have used their bioconjugation expertise to synthesise problematic biotin-antigen targets, subsequently used in the development of a number of bioanalytical assays on the Gyrolab platform
Impact of biotinylation chemistry probed in novel bioanalytical project
In collaboration with world leaders in the bioanalytical field, Fleet have investigated a range of different biotinylation chemistries and their impact on immunoassay performance. This innovative study has led to four poster presentations at international meetings and a UK government-funded follow-up project.
Routine supply of biotin-BSA reagent under GMP production conditions
Over a 20 year period, Fleet have successfully manufactured 40+ batches of a biotinylated albumin reagent to meet the needs of a major diagnostics company. The production procedure is subject to GMP documentation, which under Fleet's quality system can only be amended with the customer's written approval.