
LFA Development: Proof-of-Concept
​​​At the Proof-of-Concept (PoC) stage, the objective is to demonstrate that a proposed biological detection principle can function in a Lateral Flow Assay format. More than one assay architecture may be capable of meeting a given diagnostic aim, so this phase focuses on rapid experimental evaluation and comparison of options before progressing to full Feasibility.
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Fleet Bioprocessing Ltd’s PoC development service is designed for customers who may already have selected antibody pairs or completed antibody screening studies, as well as those who have candidate reagents but require confirmation that their concept is viable in a Lateral Flow format.
The outcome is typically a single prototype assay design that can either progress into Feasibility or be used directly to support early, exploratory clinical or research studies.
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Our PoC service offers a focused and cost effective entry point into Lateral Flow development. Get in touch to discuss how we can support your assay concept.
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Why Choose Our Proof-of-Concept LFA Service?
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Early viability check - Rapid evaluation of scientific viability in a Lateral Flow format.
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Clear development direction - Supports informed decision making before committing to feasibility.
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Format comparison - Side by side assessment of alternative assay architectures and formats.
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Novel target potential - Optimised for exploring new biomarkers & emerging detection strategies.
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Flexible approach - Adaptable to diverse exploratory projects including academia & industry.
Lateral Flow Assay Formats
Fleet Bioprocessing Ltd. has extensive experience developing PoC lateral flow assays across a wide range of formats, configurations, targets, and sample types.
​Formats:
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​​Sandwich Lateral Flow Assays - Classic detection format for proteins and analytes.
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Competitive Lateral Flow Assays - Ideal for small molecules or low molecular weight targets.
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Nucleic Acid Lateral Flow Immunoassays (NALFIA) - FITC, DIG & Biotin labels for nucleic acid detection.



​Assay Configurations:
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​Singleplex & Multiplex
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Qualitative and Quantitative
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Serological Assays
​​Target Types
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Proteins - biomarkers, antigens, enzymes
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Small molecules - drugs, metabolites, toxins
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Nucleic-acid capture formats
Sample Matrices
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Whole blood, serum and plasma
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Saliva / Nasal swabs
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Urine
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Foods






